Total RNA was extracted with the RNeasy Kit (Qiagen). After DNase I treatment, 3 ug of total RNA was used to synthesize the cDNA using iScript™ Advanced cDNA Synthesis Kit (Bio-Rad). cDNA was purified with MinElute PCR Purification Kit (Qiagen).
For the ADAR EA mutant RNAseq, total RNA was extracted using RNAdvance magnetic beads (Agencourt), treated using TURBO DNase (Thermo Fisher Scientific), depleted of ribosomal RNA [50 (link)], and treated again using TURBO DNase.
For the ADAR EA mutant RNAseq, total RNA was extracted using RNAdvance magnetic beads (Agencourt), treated using TURBO DNase (Thermo Fisher Scientific), depleted of ribosomal RNA [50 (link)], and treated again using TURBO DNase.
Free full text: Click here
