Characterization of AT/RT Cell Lines

The AT/RT cell lines CHLA-02-ATRT, CHLA-04-ATRT, and CHLA-06-ATRT have been previously described [37 (link)]. CHLA-02-ATRT and CHLA-04-ATRT are available from ATCC (Manassas, VA). The BT37 AT/RT cell line was derived from a serially passaged xenograft derived from a patient with AT/RT. Cells were grown as semi-adherent cultures in RPMI/10% FBS media (500 mL RPMI, 50 mL fetal bovine serum, 1% L-glutamine, 1% Penicillin/Streptomycin, Life Technologies, Grand Island, NY). Cells were split at high density after scraping and gentle titration. BT12 cells (available through the Children's Oncology Group cell repository, Lubbock, TX) were grown in RPMI/10% FBS media (500 mL RPMI, 50 mL fetal bovine serum, 1% L-glutamine, 1% Penicillin/Streptomycin. Selumetinib (AZD6244) was obtained from Selleck Chemicals (Houston, TX) and dissolved in DMSO.

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Weingart M.F., Roth J.J., Hutt-Cabezas M., Busse T.M., Kaur H., Price A., Maynard R., Rubens J., Taylor I., Mao X.G., Xu J., Kuwahara Y., Allen S.J., Erdreich-Epstein A., Weissman B.E., Orr B.A., Eberhart C.G., Biegel J.A, & Raabe E.H. (2014). Disrupting LIN28 in atypical teratoid rhabdoid tumors reveals the importance of the mitogen activated protein kinase pathway as a therapeutic target. Oncotarget, 6(5), 3165-3177.

Publication 2014

CHLA-02-ATRT Penicillin/Streptomycin Selumetinib (AZD6244)

Azd6244 Cell Cell line Dmso Fetal bovine serum Glutamine Oncology Patient Penicillin Selumetinib Streptomycin Titration Xenograft

Corresponding Organization :

Other organizations : Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University, Children's Hospital of Philadelphia, Children's Hospital of Los Angeles, University of North Carolina at Chapel Hill, St. Jude Children's Research Hospital, University of Southern California, University of Pennsylvania

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Variable analysis

independent variables

Selumetinib (AZD6244)

dependent variables

Cell growth/proliferation

control variables

RPMI/10% FBS media (500 mL RPMI, 50 mL fetal bovine serum, 1% L-glutamine, 1% Penicillin/Streptomycin)
Cell culture conditions (semi-adherent, split at high density after scraping and gentle titration)

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