The LAMP reaction was performed in a reaction mixture, as described previously14 (link). Briefly, the reaction mixture contained 1 × Bst DNA polymerization buffer, 1 U of Bst DNA polymerase (New England Biolabs, Frankfurt, Germany), 0.5 μM outer primers (F3 and B3 primers each), 4 μM inner primers (FIP and BIP primers each), and 200 μM dNTPs each. Finally, the different amount of sample’s genomic DNA was used to each LAMP reaction. The mixtures were reacted at 60–65 °C for 60 min in a heating block (DNA engine, Biorad, CA, USA).
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