Small RNA Detection via Northern Blot and Phosphor Imaging

Small RNAs were blotted and chemically crosslinked as described previously.^62,64 (link) For detection of miRNAs, DNA oligonucleotides were 5´-radio-labeled by T4 Polynucleotide kinase and purified by Sephadex-G50 filtration. Hybridized filters were exposed to phosphor imager plates (Fujifilm) for 5–7 d. Plates were read by the Phosphoimager FLA3000 (Fujifilm, Düsseldorf) and images analyzed with the Multigauge Software (release 3.2).

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Kruse J., Meier D., Zenk F., Rehders M., Nellen W, & Hammann C. (2016). The protein domains of the Dictyostelium microprocessor that are required for correct subcellular localization and for microRNA maturation. RNA Biology, 13(10), 1000-1010.

Publication 2016

Phosphoimager FLA3000 Multigauge Software

Filtration Mirnas Oligonucleotides Phosphor Polynucleotide kinase Rnas Sephadex

Corresponding Organization : Constructor University

Other organizations : University of Kassel

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Variable analysis

independent variables

Small RNAs were blotted and chemically crosslinked

dependent variables

Detection of miRNAs

control variables

Conditions described previously in refs. 62 and 64

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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