Poly-A selected, strand-specific RNA sequencing was performed on a CMCT surgically excised from a GR. Sequencing libraries were prepared as described [56 (link)]. Normal marginal tissue was sampled as control. Samples were sequenced on one Illumina Hi-Seq 2500 lane. Data was analysed and aligned to CanFam3.1 using the tuxedo suite [21 (link)]. The sequence data was viewed in IGV.
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