m6A Modification Detection in MYC

The methylated RNA immunoprecipitation (Me-RIP) was conducted to detect the m⁶A modification of MYC as previously described (20 (link)). In brief, total RNAs were isolated, then the mRNA was further isolated and purified using the TIANSeq mRNA Capture Kit (TIANGEN Biotech, Beijing, China). The anti-M⁶A or anti-IgG (1:100, Abcam, Cambridge, UK) were added and incubated with protein A/G agarose beads (Santa Cruz Biotechnology, Santa Cruz, CA, United States) in IP buffer overnight at 4°C. The eluent buffer was conducted to elute RNA. The phenol-chloroform was used to purify the RNAs. The qPCR was performed to determine the mRNA level of m⁶A MYC.