Apoptosis and Cell Cycle Analysis

Flow cytometry was performed using previously described methods [22 (link), 25 (link)]. HN6 and Cal27 cells transfected with si-lincRNA-p21, lincRNA-p21, or scramble were harvested 48 h after transfection. After incubating with reagents from the Annexin V-FITC/propidium iodide (PI) apoptosis kit (BD Biosciences, Franklin Lakes, NJ, USA), cells were analysed using a BD FORTASA flow cytometer (BD Biosciences) and the FlowJo software. For cell cycle analysis, cells were incubated using the PI/RNase staining kit (BD Biosciences). The subsequent steps were performed as described above.

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Jin S., Yang X., Li J., Yang W., Ma H, & Zhang Z. (2019). p53-targeted lincRNA-p21 acts as a tumor suppressor by inhibiting JAK2/STAT3 signaling pathways in head and neck squamous cell carcinoma. Molecular Cancer, 18, 38.

Publication 2019

Annexin V-FITC/propidium iodide (PI) apoptosis kit BD FORTASA flow cytometer PI/RNase staining kit

Annexin v fitc Apoptosis Cell cycle Cells Flow cytometry Lincrna Propidium iodide Rnase Transfection

Corresponding Organization :

Other organizations : Shanghai Ninth People's Hospital, Shanghai Jiao Tong University

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Variable analysis

independent variables

SiRNA transfection (si-lincRNA-p21, lincRNA-p21, or scramble)

dependent variables

Apoptosis (Annexin V-FITC/propidium iodide assay)
Cell cycle (propidium iodide/RNase staining)

control variables

Cell lines (HN6 and Cal27)

controls

Positive control: Annexin V-FITC/propidium iodide apoptosis kit
Positive control: Propidium iodide/RNase cell cycle staining kit

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