Protein Extraction and Immunoblotting Protocol

Protein extraction and immunoblotting were performed as previously described [19 (link)]. The following antibodies were used: rabbit polyclonal anti-FOXF1 (Abcam #ab23194, 1:500), rabbit monoclonal anti-p21 (Cell Signaling Technology #2947, 1:2000), mouse monoclonal anti-cyclin A2 (Cell Signaling Technology #4546, 1:1000), rabbit polyclonal anti-cyclin B1 (Cell Signaling Technology #4138, 1:1000), rabbit polyclonal anti-cyclin E2 (Cell Signaling Technology #4132, 1:750), and mouse monoclonal anti-β-actin (Millipore #MAB1501, 1:10000).

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Wu C.Y., Chan C.H., Dubey N.K., Wei H.J., Lu J.H., Chang C.C., Cheng H.C., Ou K.L, & Deng W.P. (2020). Highly Expressed FOXF1 Inhibit Non-Small-Cell Lung Cancer Growth via Inducing Tumor Suppressor and G1-Phase Cell-Cycle Arrest. International Journal of Molecular Sciences, 21(9), 3227.

Publication 2020

rabbit monoclonal anti-p21 mouse monoclonal anti-cyclin A2

Actin Antibodies Cyclin a2 Cyclin b1 Cyclin e2 Mouse Protein Rabbit

Corresponding Organization : Fu Jen Catholic University

Other organizations : Taipei Medical University Hospital, Taipei Medical University-Shuang Ho Hospital

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Protein expression levels of FOXF1, p21, cyclin A2, cyclin B1, and cyclin E2

control variables

β-actin protein expression (used as a loading control)

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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