Analysis of Thioredoxin Protein Structure

The nucleotide sequence of EpTrx was obtained from the complete genome sequence data58 (link), and its amino acid sequence was analyzed using DNAMAN. The three dimensional structure of Trx was predicted using SWISS-MODEL (http://swissmodel.expasy.org/) and software PyMOL 0.99rc6 (DeLano Scientific LLC, USA). The 3D structure of Trx from Saccharomyces cerevisiae (PDB number 2HSY) was used as a structural template. Sequences of Trx from other organisms were downloaded from Genbank for comparison (Supplementary Table S1).

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Li H, & Wei J.C. (2016). Functional analysis of thioredoxin from the desert lichen-forming fungus, Endocarpon pusillum Hedwig, reveals its role in stress tolerance. Scientific Reports, 6, 27184.

Publication 2016

PyMOL 0.99rc6

Amino acid sequence Genome Nucleotide sequence Saccharomyces cerevisiae

Corresponding Organization :

Other organizations : Chinese Academy of Sciences, University of Chinese Academy of Sciences

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Variable analysis

independent variables

Nucleotide sequence of EpTrx
Amino acid sequence of Trx
Three-dimensional structure of Trx predicted using SWISS-MODEL and PyMOL
Structural template of Trx from Saccharomyces cerevisiae (PDB number 2HSY)
Sequences of Trx from other organisms downloaded from Genbank

dependent variables

Not explicitly mentioned

control variables

Structural template of Trx from Saccharomyces cerevisiae (PDB number 2HSY)

controls

Positive control: Structural template of Trx from Saccharomyces cerevisiae (PDB number 2HSY)
Negative control: Not mentioned

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