The local collagen orientation was determined from images captured using PLM following protocols previously reported.20 (link) Briefly, a white light source and two polarizing filters were used (Hoya, Tokyo, Japan). The birefringence of the collagen fibers causes the intensity of the detected light to vary depending on the relative orientation of the fiber and the direction of polarization of the light (Fig. 1 ). Thus, local collagen orientation at each pixel can be determined from multiple images captured with different relative orientations.20 (link)
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Determining Collagen Fiber Orientation
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Corresponding Organization : University of Pittsburgh
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Variable analysis
independent variables
- Relative orientation of the collagen fibers and the direction of polarization of the light
- Intensity of the detected light
- White light source
- Two polarizing filters (Hoya, Tokyo, Japan)
- No positive or negative controls were explicitly mentioned.
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