Evaluating HNSCC Cell Behavior

Cell proliferation, migration, and invasion assays were performed using HNSCC cells. The XTT assay (Sigma–Aldrich, St. Louis, MO, USA) characterized cell proliferation, a chamber assay using the Corning BioCoat^TM cell culture chamber (Corning, Corning, NY, USA) assessed migration, and Matrigel chamber assays using the Corning BioCoat Matrigel assessed invasion. They were performed with HNSCC cells as described previously [20 (link),21 (link),57 (link),58 (link)]. The reagents used in this study are listed in Supplemental Table S1.

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Minemura C., Asai S., Koma A., Kase-Kato I., Tanaka N., Kikkawa N., Kasamatsu A., Yokoe H., Hanazawa T., Uzawa K, & Seki N. (2022). Identification of Tumor-Suppressive miR-30e-3p Targets: Involvement of SERPINE1 in the Molecular Pathogenesis of Head and Neck Squamous Cell Carcinoma. International Journal of Molecular Sciences, 23(7), 3808.

Publication 2022

XTT assay Corning BioCoatTM cell culture chamber Corning BioCoat Matrigel

Assays Cell culture Cell proliferation Cells Hnscc Matrigel

Corresponding Organization : Chiba University

Other organizations : National Defense Medical College Hospital

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Variable analysis

independent variables

Cell proliferation assay (XTT assay)
Cell migration assay (chamber assay using Corning BioCoat cell culture chamber)
Cell invasion assay (Matrigel chamber assay using Corning BioCoat Matrigel)

dependent variables

Cell proliferation
Cell migration
Cell invasion

control variables

HNSCC cells used in the assays (as described previously in references 20, 21, 57, and 58)

controls

No positive or negative controls were explicitly mentioned in the input text.

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