Lung Macrophage Analysis via Flow Cytometry

On day 12, the mice were administered FITC-labeled liposomes or blank liposomes via i.t. injection and sacrificed on day 14. Single-cell suspensions of lung tissue samples were obtained as reported previously (18 (link)). The resuspended cells were stained with stain buffer containing PE-conjugated anti-mouse F4/80 antibodies at 4°C for 30 min. Finally, the samples were washed twice with PBS prior to flow cytometry analysis. Data were acquired on a MACSQuant X (Miltenyi, Bergisch-Gladbach, Germany) and analyzed using FlowJo V10 software.

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Wu G.R., Zhou M., Wang Y., Zhou Q., Zhang L., He L., Zhang S., Yu Q., Xu Y., Zhao J., Xiong W, & Wang C.Y. (2022). Blockade of Mbd2 by siRNA-loaded liposomes protects mice against OVA-induced allergic airway inflammation via repressing M2 macrophage production. Frontiers in Immunology, 13, 930103.

Publication 2022

MACSQuant X

Anti antibodies Buffer Cells Fitc Flow cytometry Liposomes Lung Mouse Stain Tissue

Corresponding Organization : Shanghai Ninth People's Hospital

Other organizations : Shanghai East Hospital, Tongji University

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Variable analysis

independent variables

Administration of FITC-labeled liposomes or blank liposomes via i.t. injection

dependent variables

Lung tissue cell populations analyzed by flow cytometry

control variables

Cell staining with PE-conjugated anti-mouse F4/80 antibodies
Cell washing with PBS prior to flow cytometry analysis

controls

Blank liposomes as a control group

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