Vero-E6 GFP cells were seeded at 104 cells/well in 96-well black plates (PerkinElmer). The following day, cells were incubated, with or without compounds, with 2 μM P-gp inhibitor CP-100356 [47 (link)]. The media was removed 24 h after treatment, and the total well GFP fluorescence was measured using Victor NIVO 5 with 485/535 nm excitation wavelength. The cytotoxic effect of compounds was calculated as a percentage of cell viability with respect to untreated control. Data were analyzed using Prism 9. Version 9.1.2.
Messore A., Malune P., Patacchini E., Madia V.N., Ialongo D., Arpacioglu M., Albano A., Ruggieri G., Saccoliti F., Scipione L., Tramontano E., Canton S., Corona A., Scognamiglio S., Paulis A., Suleiman M., Al-Maqtari H.M., Abid F.M., Kawsar S.M., Sankaranarayanan M., Di Santo R., Esposito F, & Costi R. (2024). New Thiazolidine-4-One Derivatives as SARS-CoV-2 Main Protease Inhibitors. Pharmaceuticals, 17(5), 650.
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