Immunoblotting Assay for PMDH2 and HA Tags

Immunoblotting was done as described^{74 (link)} except that membranes were air dried for 1 h before blocking overnight in 8% (w/v) non-fat dry milk in TBST (Tris-buffered saline with 0.1% [v/v] Tween-20). Primary antibodies used were rabbit anti-PMDH2^{75 (link)} (1:5000), rat anti-HA (1:300, Roche clone 3F10, Sigma 11867423001), and mouse anti-HSC70 (1:50,000, Stressgen SPA-817). Horseradish peroxidase-conjugated secondary antibodies (1:5000 goat anti-rat IgG, Invitrogen A10549; 1:5000 goat anti-rabbit IgG, GenScript A00098; or 1:5000 goat anti-mouse IgG, GenScript A00160) were incubated for 2 h before washing in TBST. Antibodies were imaged using WesternSure Premium Chemiluminescent substrate (LI-COR Biosciences) and a LI-COR Odyssey Fc imaging system. Uncropped blots are included in the Source Data file.