Immunoblotting Techniques for Protein Analysis

SDS/PAGE and Western blotting were performed as previously described (63 (link)). The following antibodies were purchased from Thermo Fisher Scientific: Mouse anti-Puromycin (MABE343MI), Rabbit anti-Actin (PIPA1183), Mouse anti-SYNCRIP (MAB11004MI). The following antibodies were purchased from Abcam (Toronto, ON, Canada): Rabbit anti-PABP (ab21060), Rabbit anti-CSDE1 (ab201688), Rabbit anti-TRIM25 (ab167154), Rabbit anti-PABPC4 (ab220832, Abcam), Rabbit anti-PABPN1 (ab75855), Mouse anti-HRNPNK (ab39975). The following antibodies were purchased from Proteintech (Rosemont, IL, USA): Rabbit anti-SRP9 (11195-1-AP), Rabbit anti-SRP14 (11528-1-AP), Rabbit anti-STRAP (18277-1-AP). The following additional antibodies were used: Mouse anti-Tubulin (T6074, Sigma-Aldrich), Rabbit anti-RIG-I (3743S, Cell Signaling Technologies, Danvers, MA, USA), Rabbit anti-EIF3K (CSB-PA866204ESR1HU, CusaBio), and Mouse anti-DHX36 (Clone 12F33, made in-house).