Diverse Cell Lines and Viral Models for STING Pathway Research

The cell lines used for the study were iBMDC-MycSTING, THP-1, Vero cells, HEK-293T cells, HaCaT, HeLa cells, and Human foreskin fibroblasts (HFF). The choice of cell models depended on the experiments; e.g. THP-1 cells express high levels of cytokines and were used to evaluate gene expression, while HeLa cells have a large cytoplasm and were often the cell line of choice for immunofluorescence. SAVI patient-derived fibroblasts cells were obtained as described previously^{14 (link)}. Human blood-derived monocytes were isolated from normal healthy blood donor buffy coats obtained from Aarhus University Hospital Blood Bank. Human primary fibroblasts were obtained from healthy donors from Department of Dermatology and Venereology, Aarhus University Hospital. The viruses used were HSV-1 (Strain F+, and McKrae), HSV-2 (strain 333), Sendai virus (strain Cantrell). The reagents used were 2’3’-cGAMP (BIOLOG), 60mer dsDNA (DNA technology), PI(3)P diC8 (Echelon), phorbol 12-myristate 13-acetate (PMA, Sigma), Brefeldin A (BFA, Sigma), Bafilomycin A1(BafA1, InvivoGen), 3-Methyladenine (3-MA, InvivoGen), VPS34 Inhibitor 1 (VPS34 IN1, Cayman).