Mouse perfusion, preparation of frozen brain sections, and immunofluorescence staining of sections were previously described [31 (link)]. Antibodies used were rabbit anti-MAP2 for detection of dendrites (EMD Millipore, Burlington, MA, 1:150) and mouse anti-NeuN for detection of neuronal nuclei (EMD Millipore, 1:150) followed by goat-anti-rabbit IgG Alexa 488 and donkey-anti-mouse IgG Alexa 594 (ThermoFisher Scientific, Waltham, MA, 1:150), respectively. Images were captured and fluorescence signals quantified as described [31 (link)].
Protocol detail
Immunofluorescence Staining of Neuronal Markers
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Alexa 594
Anti igg
Antibodies
Brain
Dendrites
Donkey
Fluorescence
Frozen sections
Goat
Immunofluorescence
Map2
Mouse
Neuronal
Nuclei
Perfusion
Rabbit
Corresponding Organization : Icahn School of Medicine at Mount Sinai
Other organizations : Drug Discovery Laboratory (Norway), Johns Hopkins University
Top 5 similar protocols
Variable analysis
independent variables
- Mouse perfusion
- Preparation of frozen brain sections
- Immunofluorescence staining of sections
- Fluorescence signals
- Antibodies used for detection of dendrites (rabbit anti-MAP2) and neuronal nuclei (mouse anti-NeuN)
- Fluorescence detection using goat-anti-rabbit IgG Alexa 488 and donkey-anti-mouse IgG Alexa 594
- Positive control: Not specified
- Negative control: Not specified
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!