Immunohistochemistry was performed on 4-μm TMA tissue sections with a Ventana Bench Mark XT Autostainer (Ventana Medical Systems, Tucson, AZ, USA) as described in previous reports 22 (link). The following primary antibodies were tested: c-Met (pre-dilution; clone SP44; Ventana) and p16 (RTU; Ventana). c-Met protein expression was analyzed according to the semiquantitative H-score method; this method yields a total score range of 0-300 23 (link) by multiplying the dominant nuclear staining intensity score (0, no staining; 1, weak or barely detectable membranous staining; 2, distinct brown membranous staining; 3, strong dark brown membranous staining) by the percentage (0-100%) of positive cell nuclei (Figs. 1 A-D). Conventionally accepted criteria were used for p16 immunohistochemistry, and positivity was defined as the presence of strong and diffuse nuclear and cytoplasmic staining in >70% of the HNSCC cells. All other staining patterns were scored as negative 24 (link).
Protocol detail
Tissue Microarray Immunohistochemistry Analysis
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
A 300
Antibodies
C met
C protein
Cell nuclei
Cells
Clone
Cytoplasmic
Dilution
Figs
Hnscc
Immunohistochemistry
Membranous
Tissue
Weak
Corresponding Organization : Yonsei University
Top 5 similar protocols
Protocol cited in 1 other protocol
Variable analysis
independent variables
- Primary antibodies tested: c-Met (pre-dilution; clone SP44; Ventana) and p16 (RTU; Ventana)
- C-Met protein expression analyzed according to the semiquantitative H-score method (score range of 0-300)
- P16 immunohistochemistry positivity defined as the presence of strong and diffuse nuclear and cytoplasmic staining in >70% of the HNSCC cells
- Tissue sections: 4-μm TMA tissue sections
- Autostainer: Ventana Bench Mark XT Autostainer (Ventana Medical Systems, Tucson, AZ, USA)
- No explicit mention of positive or negative controls
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!