Validating MTBC Nested PCR Specificity
Corresponding Organization :
Other organizations : Chulalongkorn University, National Science and Technology Development Agency
Variable analysis
- Concentration of M. tuberculosis H37Rv genomic DNA (1 ng to 1 fg)
- Sensitivity of the M. tuberculosis nested PCR technique (lowest concentration detected)
- Granuloma lung tissues from a naturally M. tuberculosis-infected long-tailed macaque
- Mycobacterium culture and interferon gamma release assay for confirming M. tuberculosis infection in the macaque
- QIAGEN Virus/Pathogen Mini Kit for genomic DNA extraction from granuloma lung tissues
- GenUP Exo Sap Kit for PCR product purification
- Alignment of nucleotide sequences with published M. tuberculosis sequences from GenBank using MEGA X software
- Genomic DNA of the M. tuberculosis H37Rv (ATCC27294) strain
- Not explicitly mentioned
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