All procedures described below were approved by The Institutional Animal Care and Use Committee at Johns Hopkins University. Individual Myomatrix threads were implanted on the masseter muscle using the “epimysial” method described above. A ground pin was placed over the right visual cortex. As described previously (Severson et al. 2017 (link)), EMG signals and high-speed video of the orofacial area were recorded simultaneously in head-fixed animals under isoflurane anesthesia (0.9–1.5% at flow rate 1L/min). During data collection, the experimenter used a thin wooden dowel to gently displace the mandible to measure both jaw displacement and muscle activity from the jaw jerk reflex. Jaw kinematics were quantified using a high-speed camera (PhotonFocus DR1-D1312-200-G2-8) at 400 frames per second using an angled mirror to collect side and bottom views simultaneously. Jaw displacement was quantified by tracking eleven keypoints along the jaw using DeepLabCut (Mathis et al. 2018 (link)).
Chung B., Zia M., Thomas K.A., Michaels J.A., Jacob A., Pack A., Williams M.J., Nagapudi K., Teng L.H., Arrambide E., Ouellette L., Oey N., Gibbs R., Anschutz P., Lu J., Wu Y., Kashefi M., Oya T., Kersten R., Mosberger A.C., O’Connell S., Wang R., Marques H., Mendes A.R., Lenschow C., Kondakath G., Kim J.J., Olson W., Quinn K.N., Perkins P., Gatto G., Thanawalla A., Coltman S., Kim T., Smith T., Binder-Markey B., Zaback M., Thompson C.K., Giszter S., Person A., Goulding M., Azim E., Thakor N., O’Connor D., Trimmer B., Lima S.Q., Carey M.R., Pandarinath C., Costa R.M., Pruszynski J.A., Bakir M, & Sober S.J. (2023). Myomatrix arrays for high-definition muscle recording. bioRxiv.
Other organizations :
Georgia Institute of Technology, Western University, Columbia University, Champalimaud Foundation, Otto-von-Guericke University Magdeburg, Tufts University, Johns Hopkins University, Johns Hopkins Medicine, University Hospital Cologne, Salk Institute for Biological Studies, University of Colorado Anschutz Medical Campus, Drexel University, Temple University, Allen Institute
Isoflurane anesthesia (0.9–1.5% at flow rate 1L/min)
Head-fixed animals
controls
Ground pin placed over the right visual cortex
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