Murine Cancer Cell Line Propagation

The 4T1 murine breast cancer cell line was purchased from ATCC. The B78-D14 cell line was derived from B16 melanoma and was obtained from Dr. Ralph Reisfield (Scripps Research Institute). The NXS2 cell murine neuroblastoma cell line was obtained from Dr. Alice Yu. B16 Tmem173 −/− CRISPR KO and corresponding wild type (WT) melanoma cells were obtained from Dr. Samuel Bakhoum (Memorial Sloan Kettering Cancer Center). Panc02 pancreatic cancer line was obtained from NCI. B78, 4T1, NXS2, Panc02, and B16 cells were grown in RPMI 1640 or DMEM (Mediatech) media and supplemented with 10% fetal bovine serum (FBS), 2 mM L- glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin. Cells were maintained in culture below 80% confluence for all passages, and early passages after thaw (3 (link)–8 (link)) were used for all experiments. Cell authentication was performed per ATCC guidelines using morphology, growth curves, and mycoplasma testing within 6 months of use.

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Patel R.B., Hernandez R., Carlson P., Grudinski J., Bates A.M., Jagodinsky J.C., Erbe A., Marsh I.R., Arthur I., Aluicio-Sarduy E., Sriramaneni R., Jin W.J., Massey C., Rakhmilevich A.L., Vail D., Engle J.W., Le T., Kim K., Bednarz B., Sondel P.M., Weichert J, & Morris Z.S. (2021). Low-dose targeted radionuclide therapy renders immunologically “cold” tumors responsive to immune checkpoint blockade. Science translational medicine, 13(602), eabb3631.

Publication 2021

4T1 murine breast cancer cell line

B16 melanoma Breast cancer cell line Cancer Cell Cell line Crispr Fetal bovine serum Glutamine Melanoma Murine Mycoplasma Neuroblastoma Pancreatic cancer Penicillin Streptomycin

Corresponding Organization : University of Wisconsin–Madison

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Variable analysis

independent variables

Cell lines: 4T1 murine breast cancer, B78-D14 (derived from B16 melanoma), NXS2 murine neuroblastoma, B16 Tmem173 −/− CRISPR KO and corresponding wild type (WT) melanoma, Panc02 pancreatic cancer

dependent variables

Not explicitly mentioned

control variables

Cell growth media: RPMI 1640 or DMEM, supplemented with 10% fetal bovine serum (FBS), 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin
Cell culture conditions: maintained below 80% confluence for all passages, and early passages after thaw (3-8) were used for all experiments
Cell authentication: performed per ATCC guidelines using morphology, growth curves, and mycoplasma testing within 6 months of use

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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