Whole Genome Sequencing of CTX-Resistant Isolates

Two isolates (JNM56C1 and JNM60C2) were subcultured in the presence of 30 μg/mL CTX. Total DNA from both were purified using the QIAamp DNA extraction mini kit (Qiagen, Hilden, Germany) and were subjected to paired end whole genome sequencing (2 × 300 bp) on an Illumina HiSeq2500 platform (Illumina, San Diego, CA, USA). Both de novo and reference guided assembly was carried out using Velvet and Bowtie2, respectively [49 (link),50 (link)], to build genomes as described previously [51 (link)].

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Chakraborty M., Bardhan T., Basu M, & Bhattacharjee B. (2022). Influence of Sub-Inhibitory Dosage of Cefotaxime on Multidrug Resistant Staphylococcus haemolyticus Isolated from Sick Neonatal Care Unit. Antibiotics, 11(3), 360.

Publication 2022

QIAamp DNA extraction mini kit HiSeq2500 platform

Genomes

Corresponding Organization : National Institute of Biomedical Genomics

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Variable analysis

independent variables

Presence of 30 μg/mL CTX

dependent variables

Total DNA from both isolates (JNM56C1 and JNM60C2)

control variables

Not explicitly mentioned

controls

No positive or negative controls were explicitly mentioned.

Annotations

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