Human hypopharyngeal carcinoma cell, FaDu, was purchased from the American Type Culture Collection (Manassas, VA, USA) and cultured in Dulbecco’s modified Eagle’s medium with 10% fetal bovine serum (Gibco-BRL, Carlsbad, CA, USA) and penicillin-streptomycin at 37°C incubator according to previous study [15 (link)]. Human hypopharyngeal primary cells (HHPCs) were purchased from Celprogen Inc. (Carlsbad, CA, USA) and cultured in Human Hypopharyngeal Normal Cell Culture Media with Serum (Celprogen) according to previous study [16 (link)].
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