Unilateral 6-OHDA Lesion Protocol for Parkinson's Disease
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Corresponding Organization :
Other organizations : Medical Research Council, University of Oxford, Sapientia Hungarian University of Transylvania
Protocol cited in 11 other protocols
Variable analysis
- Unilateral 6-OHDA lesions
- Extent of the dopamine neuron lesion assessed by challenge with apomorphine (0.05 mg/kg, s.c.)
- Emergence of exaggerated β oscillations
- Rats weighing 190–305 g
- Desipramine (25 mg/kg, i.p.) administered 25 min before 6-OHDA injection to minimize uptake by noradrenergic neurons
- Anesthesia induced and maintained with isoflurane
- 6-OHDA (4 mg/ml) dissolved in 0.9% w/v NaCl solution containing 0.02% w/v ascorbate
- 6-OHDA injected into the region adjacent to the medial substantia nigra (4.5 mm posterior and 1.2 mm lateral of bregma, and 7.9 mm ventral to the dura)
- Electrophysiological recordings performed ipsilateral to 6-OHDA lesions in anesthetized rats 21–45 d after surgery
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