Mitochondrial Respiration Profiling in LCLs

We used the state-of-the-art Seahorse Extracellular Flux (XF) 96 Analyzer (Seahorse Bioscience, North Billerica, MA, USA) to measure oxygen consumption rate, an indicator of mitochondrial respiration, in real-time in live intact LCLs. Each run contained the three matched samples (control, AD-N and AD-A) on the same plate. The Seahorse assay (Supplementary Figure 1A), described previously,^{67 (link), 68 (link)} provides measures of ATP-linked respiration, proton leak respiration, maximal respiratory capacity and reserve capacity.

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Frye R.E., Rose S., Chacko J., Wynne R., Bennuri S.C., Slattery J.C., Tippett M., Delhey L., Melnyk S., Kahler S.G, & MacFabe D.F. (2016). Modulation of mitochondrial function by the microbiome metabolite propionic acid in autism and control cell lines. Translational Psychiatry, 6(10), e927-.

Publication 2016

Seahorse Extracellular Flux (XF) 96 Analyzer

Assay Lcls Mitochondrial Oxygen consumption Proton Respiration Respiratory Seahorse

Corresponding Organization : University of Arkansas for Medical Sciences

Other organizations : Arkansas Children's Hospital, Western University

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Protocol cited in 2 other protocols

Variable analysis

independent variables

Treatment conditions (control, AD-N, AD-A)

dependent variables

Oxygen consumption rate (indicator of mitochondrial respiration)

control variables

Matched samples (control, AD-N and AD-A) on the same plate

controls

Positive control: Not explicitly mentioned.
Negative control: Not explicitly mentioned.

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