Transcriptomic Analysis of HULK Gene Expression

Analysis of HULK gene expression pattern by means of RNA-Seq, in situ hybridization and GUS staining are described in Methods S2 (Weigel and Glazebrook, 2002 ; Kover et al., 2009 (link); Gan et al., 2011 (link); Trapnell et al., 2012 (link)).
Transcriptome profiling using Illumina RNA-Seq (http://www.illumina.com/) with biological replication for seedlings of Col–0, hua2–7 single mutants, hua2–7 hulk1 double mutants and hua2–7 hulk1 hulk2 triple mutants is described in Methods S3 (Anders and Huber, 2010 (link); Doherty and Kay, 2010 (link); Du et al., 2010 ; Gan et al., 2011 (link); Trapnell et al., 2012 (link)).

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Jali S.S., Rosloski S.M., Janakirama P., Steffen J.G., Zhurov V., Berleth T., Clark R.M, & Grbic V. (2014). A plant-specific HUA2-LIKE (HULK) gene family in Arabidopsis thaliana is essential for development. The Plant Journal, 80(2), 242-254.

Publication 2014

RNA-Seq

Biological In situ hybridization Replication Rna seq Seedlings

Corresponding Organization : University of Utah

Other organizations : University of Toronto

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Variable analysis

independent variables

Genotypes: Col-0, hua2-7 single mutants, hua2-7 hulk1 double mutants, hua2-7 hulk1 hulk2 triple mutants

dependent variables

HULK gene expression pattern analyzed by RNA-Seq, in situ hybridization, and GUS staining

control variables

Biological replicates for each genotype

controls

No positive or negative controls were explicitly mentioned in the provided information.

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