Protocol detail

Immortalized Head and Neck PGL Cell Lines

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We have previously established and immortalized head and neck PGL cell cultures PTJ64i and PTJ86i from two patients carrying the SDHC c.43 C > T (p.Arg15*) and SDHD c.27delC (p.Val10Phefs*5) mutations, respectively^{9 (link)}. PTJ64i and PTJ86i cell lines were cultured in DMEM-F12 (Sigma), supplemented with 10% FBS, at 37 °C, 5% CO₂. Sodium dichloroacetate (DCA) and metformin were purchased from Sigma Aldrich. GW6471 was obtained from Tocris Bioscience (Bristol, UK).

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Florio R., De Lellis L., Veschi S., Verginelli F., di Giacomo V., Gallorini M., Perconti S., Sanna M., Mariani-Costantini R., Natale A., Arduini A., Amoroso R., Cataldi A, & Cama A. (2018). Effects of dichloroacetate as single agent or in combination with GW6471 and metformin in paraganglioma cells. Scientific Reports, 8, 13610.

Publication 2018

DMEM-F12 Sodium dichloroacetate (DCA) metformin GW6471

Cell cultures Cell lines Gw6471 Head Metformin Mutations Neck Patients Sdhc Sodium dichloroacetate

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Variable analysis

independent variables

Sodium dichloroacetate (DCA)
Metformin
GW6471

dependent variables

Measured outcomes not explicitly mentioned

control variables

Cell culture conditions: DMEM-F12 (Sigma), supplemented with 10% FBS, at 37 °C, 5% CO2

controls

Positive control: Not specified
Negative control: Not specified

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