Rabbit polyclonal anti-vps35 antibody was generated by using the antigen of GST–VPS35D1 fusion protein as described [14 (link), 17 (link)]. Rabbit polyclonal anti-Synapsin 1a/1b (Santa Cruz), GluR1 (Millipore), GFP (Santa Cruz) antibodies and mouse monoclonal anti-PSD95 (Millipore), GluR1 (Millipore), GluR2 (Millipore), β-actin (Upstate), Flag (Sigma), and TfR (Abcam) antibodies were used.
VPS35 mutant mice were generated by injection of mutant embryonic stem (ES) cells obtained from Bay Genomics as described previously [17 (link), 20 (link)]. All experimental procedures were approved by the Animal Subjects Committee at the Georgia Regents University, according to US National Institutes of Health guidelines.
VPS35 mutant mice were generated by injection of mutant embryonic stem (ES) cells obtained from Bay Genomics as described previously [17 (link), 20 (link)]. All experimental procedures were approved by the Animal Subjects Committee at the Georgia Regents University, according to US National Institutes of Health guidelines.
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