Leaf Carbon and Nitrogen Analysis

Leaflet samples were analysed for nitrogen and carbon content (C) and carbon isotope discrimination (δ¹³C) following Krieg et al. (2017) (link) using mass spectrometry facilities in the Department of Biology at Colgate University. Leaflet samples were oven dried at 65°C for 72 hours and ground into a powder using a Wiley Mill (Thomas Scientific Model 3383-L10). Ground samples were then weighed and rolled into tin capsules. Rolled samples were run through an elemental analyser, Costech Instruments Elemental Combustion System (Costech Analytical Technologies Inc. Valencia, CA, USA) in tandem with a Delta Plus Advantage Stable Isotope Mass Spectrometer (Thermo Fisher Scientific Inc. Waltham, MA, USA). Leaf isotope values are expressed in delta notation (‰) relative to the standard Pee Dee Belemnite and air, for C and N, respectively. Photosynthetic nitrogen-use efficiency (PNUE) was calculated by dividing leaf-level maximum photosynthetic rate by leaf nitrogen content.

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Krieg C.P., Gosetti S., Watkins Jr J.E., Griffith M.P, & McCulloh K.A. (2023). Reproductive phases coincide with changes in morphology and photosynthetic physiology in an endangered cycad species. Conservation Physiology, 11(1), coad020.

Publication 2023

Elemental Combustion System Delta Plus Advantage Stable Isotope Mass Spectrometer

Capsules Carbon Carbon isotope Discrimination Isotope Leaf Mass spectrometry Nitrogen Photosynthetic Powder

Corresponding Organization :

Other organizations : Colgate University, Montgomery Botanical Center

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Nitrogen content (N)
Carbon content (C)
Carbon isotope discrimination (δ^13C)
Photosynthetic nitrogen-use efficiency (PNUE)

control variables

Leaf samples were oven dried at 65°C for 72 hours
Leaf samples were ground into a powder using a Wiley Mill (Thomas Scientific Model 3383-L10)
Rolled samples were run through an elemental analyser, Costech Instruments Elemental Combustion System (Costech Analytical Technologies Inc. Valencia, CA, USA) in tandem with a Delta Plus Advantage Stable Isotope Mass Spectrometer (Thermo Fisher Scientific Inc. Waltham, MA, USA)

controls

Positive control: Not mentioned
Negative control: Not mentioned

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