Breastmilk Carotenoid Profiling by HPLC

Carotenoid analyses were conducted using the high-performance liquid chromatography (HPLC) method as detailed previously [41 (link)]. Before analysis, the breastmilk samples were saponified based on the modified method by Macias and Schweigert [43 (link)]. The concentrations of selected carotenoids (β-carotene, lycopene and lutein + zeaxanthin) were determined using a Shimadzu HPLC system (Japan: 2 LC-20AD pumps, CMB-20A controller system, SIL-20AC autosampler, UV/IS SPD-20AV detector, CTD-20AC controller) using C18 Synergi Fusion-RP 80i columns (250 × 4.60 mm, Phenomenex, CA, USA). The concentration of carotenoids was assessed based on standard curves prepared with Sigma Aldrich standards (catalogue numbers: β-carotene C4582, lutein X6250, lycopene L9879, Merck KGaA, Darmstadt, Germany) and expressed in nmol/L.

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Zielinska M.A., Hamulka J., Grabowicz-Chądrzyńska I., Bryś J, & Wesolowska A. (2019). Association between Breastmilk LC PUFA, Carotenoids and Psychomotor Development of Exclusively Breastfed Infants. International Journal of Environmental Research and Public Health, 16(7), 1144.

Publication 2019

HPLC system C18 Synergi Fusion-RP 80i columns

Breastmilk Carotene Carotenoids High performance liquid chromatography Lutein Lycopene Zeaxanthin

Corresponding Organization :

Other organizations : Warsaw University of Life Sciences, Medical University of Warsaw

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Variable analysis

independent variables

Saponification method used to prepare the breastmilk samples

dependent variables

Concentrations of selected carotenoids (β-carotene, lycopene and lutein + zeaxanthin)

control variables

HPLC method and system used for carotenoid analyses, including the Shimadzu HPLC system components and C18 Synergi Fusion-RP 80i columns
Standard curves prepared with Sigma Aldrich standards for quantifying carotenoid concentrations

controls

Positive control: Sigma Aldrich standards for β-carotene, lutein, and lycopene
Negative control: Not explicitly mentioned

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