Lipopolysaccharide Extraction and Phage Inactivation

LPS extraction from A. xylosoxidans was performed using an LPS extraction kit from Intron Biotechnology (17144; Boca Scientific, Boca Raton, FL, USA), according to the manufacturer’s instructions. LPS from Escherichia coli O111:B4 purchased from Sigma-Aldrich, Inc. (L2630; Sigma, USA) was used as a negative control to ensure that the possible effect was specific to A. xylosoxidans LPS. Both LPS of Escherichia coli O111:B4 and A. xylosoxidans A22732 are smooth type. The phage inactivation by LPS was performed as previously described34 (link).

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Li E., Zhao J., Ma Y., Wei X., Li H., Lin W., Wang X., Li C., Shen Z., Zhao R., Jiang A., Yang H., Yuan J, & Zhao X. (2016). Characterization of a novel Achromobacter xylosoxidans specific siphoviruse: phiAxp-1. Scientific Reports, 6, 21943.

Publication 2016

LPS extraction kit

Boca Escherichia coli Intron Phage

Corresponding Organization :

Other organizations : South China Agricultural University, Fifth Affiliated Hospital of Zhengzhou University, Henan Institute of Science and Technology, Academy of Military Medical Sciences

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Variable analysis

independent variables

LPS extraction from A. xylosoxidans
LPS from Escherichia coli O111:B4

dependent variables

Phage inactivation by LPS

control variables

LPS from Escherichia coli O111:B4 used as a negative control

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