Antiviral Drug Treatment Evaluation in Cardiac Myocytes

Cultures were treated with or without 5 μM chloroquine (Sigma; #C6628) (21 (link)), 5 μM hydroxychloroquine (Sigma; # H0915), 5 μM remdesivir (Biosynth Carbosynth; AG170167), 25 μM lopinavir/ritonavir (Sigma; #SML1222-10MG, #SML0491-10MG), and lopinavir/ritonavir/8 U interferon-β (Sigma; #IF014) for 24 h. After treatment, cells were analyzed as described below. Drug concentrations chosen are based on literature data and are below the 50% cytotoxic concentrations (22 (link), 23 (link)). Incubation of cardiac myocytes with drugs for 24 h under normal culture conditions did not result in cytotoxicity.

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Jakobi T., Groß J., Cyganek L, & Doroudgar S. (2022). Transcriptional Effects of Candidate COVID-19 Treatments on Cardiac Myocytes. Frontiers in Cardiovascular Medicine, 9, 844441.

Publication 2022

chloroquine hydroxychloroquine remdesivir lopinavir/ritonavir

Cardiac myocytes Cells Chloroquine Cytotoxicity Drugs Hydroxychloroquine Interferon β Lopinavir ritonavir Remdesivir

Corresponding Organization : University of Arizona

Other organizations : University Hospital Heidelberg, German Centre for Cardiovascular Research

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Variable analysis

independent variables

5 μM chloroquine (Sigma; #C6628)
5 μM hydroxychloroquine (Sigma; # H0915)
5 μM remdesivir (Biosynth Carbosynth; AG170167)
25 μM lopinavir/ritonavir (Sigma; #SML1222-10MG, #SML0491-10MG)
Lopinavir/ritonavir/8 U interferon-β (Sigma; #IF014)

dependent variables

Measured outcomes after treatment

control variables

Cultures treated without any drug

controls

Positive control: Not specified
Negative control: Cultures treated without any drug

Annotations

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