Quantifying Worm Crawling Speed

Automated tracking was performed as described [18 (link)]. For each recording session, 10–15 iL3s were placed on a chemotaxis plate and allowed to acclimate for 10 minutes. iL3 movement was then captured for 20 seconds using an Olympus E-PM1 digital camera attached to a Leica S6 D microscope. WormTracker and WormAnalyzer [35 (link)] were used to quantify crawling speed. WormTracker and WormAnalyzer settings were previously described [18 (link)].

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Ruiz F., Castelletto M.L., Gang S.S, & Hallem E.A. (2017). Experience-dependent olfactory behaviors of the parasitic nematode Heligmosomoides polygyrus. PLoS Pathogens, 13(11), e1006709.

Publication 2017

E-PM1 S6 D microscope

Camera Chemotaxis Microscope Movement Seconds

Corresponding Organization : University of California, Los Angeles

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Variable analysis

independent variables

Automated tracking performed as described [18 (link)]

dependent variables

Crawling speed of iL3s (quantified using WormTracker and WormAnalyzer [35 (link)])

control variables

Number of iL3s placed on the chemotaxis plate (10-15)
Acclimation time (10 minutes)
Recording duration (20 seconds)
Camera (Olympus E-PM1 digital camera)
Microscope (Leica S6 D)
WormTracker and WormAnalyzer settings (previously described [18 (link)])

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