Changes in the morphology of NIH3T3 cells expressing novel mTOR mutants were determined as described previously [42 (link),12 (link)]. Briefly, NIH3T3 cells stably transfected with the empty vector, wild-type mTOR, and the indicated mTOR mutants were plated in 60-mm culture plates (Corning, NY) at a density of 3 X 105 cells. Cells were cultivated one day in regular medium (DMEM+10% FCS+G418 800µg/ml), followed by examination of the cell morphology under a microscope (Nikon Eclipse Ti-U, Tokyo, Japan).
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Morphological Changes in NIH3T3 Cells Expressing mTOR Mutants
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Corresponding Organization :
Other organizations : Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University
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Variable analysis
independent variables
- MTOR mutants
- Morphology of NIH3T3 cells
- Empty vector
- Wild-type mTOR
- Positive control: Wild-type mTOR
- Negative control: Empty vector
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