Primary human umbilical vein endothelial cells (HUVECs) were cultured at 37 °C and 5% CO2 in a humidified incubator in the endothelial cell growth basal medium-2 supplemented with 2% fetal bovine serum and growth supplements (Lonza, Basel, Switzerland). HUVEC passages between 4 and 8 were used in the present study. Murine brain endothelial cells were isolated from 8- to 10-week-old Gab2−/− mice and WT (wild type) littermate controls using the procedure described in our recent publication.34 (link)
Protocol detail
Culturing Primary Endothelial Cells
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Brain
Endothelial cells
Fetal bovine serum
Gab2
Growth medium
Human umbilical vein endothelial cells
Mice
Supplements
Corresponding Organization :
Other organizations : The University of Texas Health Science Center at Tyler
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Variable analysis
independent variables
- Mouse genotype (Gab2-/- and wild type)
- Properties and characteristics of murine brain endothelial cells
- Age of mice (8- to 10-week-old)
- Cell culture conditions (37 °C, 5% CO2, humidified incubator)
- Culture medium (endothelial cell growth basal medium-2 supplemented with 2% fetal bovine serum and growth supplements)
- HUVEC passage number (4 to 8)
- Wild type (WT) littermate controls for Gab2-/- mice
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