Serum AOPP Concentration Measurement

Serum AOPP concentration was measured by spectrophotometry as previously described [44 (link)]. Assays of AOPP in sera were diluted (1:5) in PBS and distributed (200 μL) onto a 96-well plate with 10 μL of 1.16 M potassium iodide. Calibration used a twofold dilution series of chloramine-T solution within a range of 0 to 100 mM. The absorbance was read at 340 nm on a microplate reader (Fusion; PerkinElmer, Wellesley, MA, USA), and AOPP concentration was expressed as mM of chloramine-T equivalents.

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You F., Nicco C., Harakawa Y., Yoshikawa T, & Inufusa H. (2024). The Potential of Twendee X® as a Safe Antioxidant Treatment for Systemic Sclerosis. International Journal of Molecular Sciences, 25(5), 3064.

Publication 2024

Corresponding Organization :

Other organizations : Gifu University, Louis Pasteur Center for Medical Research, Sorbonne Paris Cité, Université Paris Cité, Kyoto Prefectural University of Medicine

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

AOPP concentration

control variables

Dilution of sera samples (1:5) in PBS
Volume of sera samples and potassium iodide (200 μL and 10 μL, respectively)
Absorbance read at 340 nm on a microplate reader
Calibration using a twofold dilution series of chloramine-T solution within a range of 0 to 100 mM

controls

Positive control: Chloramine-T solution dilution series (0 to 100 mM)
Negative control: Not explicitly mentioned

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