HUVECs were stained with 2 μM boron-dipyrromethene (BODIPY) 493/50317 (link) (D3922; Thermo Fisher) for 15 min. Then, cells were washed with PBS and analysed by flow cytometry.
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Protocol detail
BODIPY 493/503 staining of HUVECs
Corresponding Organization : The University of Tokyo
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Variable analysis
independent variables
- Staining with 2 μM boron-dipyrromethene (BODIPY) 493/503
- Flow cytometry analysis
- Incubation time of 15 minutes
- Washing cells with PBS
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