In vivo and ex vivo BLI was performed on IVIS50 (PerkinElmer, Waltham, MA) as previously described (20 (link)). Total photon flux (photons/sec) was measured from fixed regions of interest (ROIs) over entire mouse or manually around ex vivo organs using Living Image 2.6, as indicated. Mice with outstanding chest BLI intensity indicative of a failed intracardiac injection or with ineffective D-luciferin administration were excluded from all analyses. Investigators were blinded to treatment groups during all BLI analyses.
Protocol detail
In vivo and ex vivo Bioluminescence Imaging
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Corresponding Organization : Washington University in St. Louis
Other organizations : University of Illinois Urbana-Champaign, Pennsylvania State University, Indiana University – Purdue University Indianapolis
Top 5 similar protocols
Variable analysis
independent variables
- Treatment groups
- Total photon flux (photons/sec)
- Chest BLI intensity indicative of a failed intracardiac injection
- Ineffective D-luciferin administration
- Mice with outstanding chest BLI intensity indicative of a failed intracardiac injection or with ineffective D-luciferin administration were excluded from all analyses.
- Investigators were blinded to treatment groups during all BLI analyses.
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!