Antimicrobial Susceptibility of P. putida

The agar disk diffusion test was performed for all the recovered P. putida isolates (n = 18) according to the method described by Ali et al. [35 ]. Susceptibility for florfenicol (10 µg), ampicillin (10 µg), erythromycin (15 µg), sulfamethoxazole-trimethoprim (25 µg), ciprofloxacin (5 µg), doxycycline (30 µg), and tylosin (15 µg), Oxoid, UK. Muller-Hinton agar plates (Oxoid, Uk) were incubated at 37 °C for 48 h due to the slow growth rate of the tested isolates. The inhibition zone (mm) was measured to the nearest mm and interpreted according to the breakpoints mentioned [36 (link)].

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Aboyadak I.M., Abdel-Tawwab M, & Ali N.G. (2024). Identification and florfenicol-treatment of pseudomonas putida infection in gilthead seabream (Sparus aurata) fed on tilapia-trash-feed. BMC Veterinary Research, 20, 156.

Publication 2024

florfenicol ampicillin erythromycin sulfamethoxazole ciprofloxacin doxycycline Muller-Hinton agar plates

Corresponding Organization :

Other organizations : National Institute of Oceanography and Fisheries, Central Laboratory for Agricultural Climate, Agricultural Research Center

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Variable analysis

independent variables

Antimicrobial agents: florfenicol (10 µg), ampicillin (10 µg), erythromycin (15 µg), sulfamethoxazole-trimethoprim (25 µg), ciprofloxacin (5 µg), doxycycline (30 µg), and tylosin (15 µg)

dependent variables

Inhibition zone (mm) for each antimicrobial agent

control variables

Muller-Hinton agar plates
Incubation at 37 °C for 48 h

controls

No positive or negative controls were explicitly mentioned.

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