Nitric Oxide Metabolite Quantification by Griess Assay

Griess method was used to measure plasma concentration of NO metabolites as described previously [49 (link)]. Briefly, plasma samples and standards (sodium nitrite, Sigma) were mixed with N-(1-Naphthyl) ethylenediamine dihydrochloride (NEDD, Sigma), sulfanilamide (SULF, Sigma), and vanadium chloride (Sigma) and incubated at 37 °C for 30 min. Then, 100 μl of prepared solutions was added to each well, and the absorbance of well-plate was detected at 540 nm.

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Sepehrinezhad A., Dehghanian A., Rafati A, & Ketabchi F. (2020). Impact of liver damage on blood-borne variables and pulmonary hemodynamic responses to hypoxia and hyperoxia in anesthetized rats. BMC Cardiovascular Disorders, 20, 13.

Publication 2020

sodium nitrite vanadium chloride

Chloride Ethylenediamine dihydrochloride Plasma Sodium nitrite Sulfanilamide Vanadium

Corresponding Organization :

Other organizations : Shiraz University of Medical Sciences

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Variable analysis

independent variables

Concentration of sodium nitrite (standard)

dependent variables

Plasma concentration of NO metabolites

control variables

Incubation temperature (37 °C)
Incubation time (30 minutes)
Reagents used (NEDD, SULF, vanadium chloride)

controls

Positive control: Sodium nitrite (Sigma) standards
Negative control: Not explicitly mentioned

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