A total of 5 different sections and 10 areas of view were randomly examined for every patient of CVD and HC groups. A patient was reported as positive when the stained mean area in the studied sample was ≥5% of the total, following the immunoreactive score (IRS) as established in previous studies [22 (link)]. Immunostaining was evaluated by two independent histologists, and then each sample was scored using the following scale: 0–1, minimum staining (≤25%); 2, moderate staining (25–65%); and 3–4, strong staining (≥65–100%). A Zeiss Axiophot optical microscope (Carl Zeiss, Oberkochen, Germany) was used for the histological determination.
For the treatment of statistical data, GraphPad Prism® v6.0 (GraphPad, Inc., San Diego, CA, USA) program was utilized. Mann–Whitney U test allow the comparison between both groups, being expressed as the median ± SEM. Significant values were established as p < 0.05 (*), p < 0.01 (**), and p < 0.001 (***).
For the treatment of statistical data, GraphPad Prism® v6.0 (GraphPad, Inc., San Diego, CA, USA) program was utilized. Mann–Whitney U test allow the comparison between both groups, being expressed as the median ± SEM. Significant values were established as p < 0.05 (*), p < 0.01 (**), and p < 0.001 (***).
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