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Expression and Purification of SARS-CoV-2 Spike Proteins

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Esposito D., Mehalko J., Drew M., Snead K., Wall V., Taylor T., Frank P., Denson J.P., Hong M., Gulten G., Sadtler K., Messing S, & Gillette W. (2020). Optimizing high-yield production of SARS-CoV-2 soluble spike trimers for serology assays. Protein Expression and Purification, 174, 105686.

Publication 2020

Plasmid Plus Maxi Kit

Amino acids Cleavage Fargo Furin His6 tag Mers cov Mutations Phage t4 Plasmid Proline Protein signal sequence Rhinovirus 3c protease Sars cov 2 Sars cov spike proteins Spike protein Transfection Vaccine

Corresponding Organization : Protein Express (United States)

Other organizations : National Institutes of Health, National Institute of Biomedical Imaging and Bioengineering

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