Longitudinal Evaluation of Tuberculosis Progression

CT scans were collected from sedated animals using a 16-slice Lightspeed CT scanner (General Electric Healthcare, Milwaukee, WI, USA) 6, 15, 18, 24 and 32 weeks after aerosol exposure to M. tb, as described previously [23 (link)]. Scans were evaluated by an expert thoracic radiologist blinded to the animal’s treatment and clinical status for the number and distribution across lung lobes of pulmonary lesions and the presence of extrapulmonary disease [17 (link)]. The disease burden attributable to infection with M. tb was scored using a relative scoring system based on the number of lesions present in lungs, spleen, liver, kidney and lymph nodes and the presence and extent of TB-induced structural abnormalities, as described previously [17 (link)].

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White A.D., Sibley L., Gullick J., Sarfas C., Clark S., Fagrouch Z., Verschoor E., Salguero F.J., Dennis M, & Sharpe S. (2021). TB and SIV Coinfection; a Model for Evaluating Vaccine Strategies against TB Reactivation in Asian Origin Cynomolgus Macaques: A Pilot Study Using BCG Vaccination. Vaccines, 9(9), 945.

Publication 2021

Lightspeed CT scanner

Abnormalities Animal Ct scanner Ct scans Electric Infection Kidney Liver Lungs Lymph nodes Radiologist Scans Spleen

Corresponding Organization : Public Health England

Other organizations : Biomedical Primate Research Centre

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Variable analysis

independent variables

Time after aerosol exposure to M. tb (6, 15, 18, 24, and 32 weeks)

dependent variables

Number and distribution of pulmonary lesions across lung lobes
Presence of extrapulmonary disease
Disease burden attributable to infection with M. tb (scored using a relative scoring system based on the number of lesions present in lungs, spleen, liver, kidney and lymph nodes and the presence and extent of TB-induced structural abnormalities)

control variables

Use of a 16-slice Lightspeed CT scanner (General Electric Healthcare, Milwaukee, WI, USA) for collecting CT scans
Sedated animals
Expert thoracic radiologist blinded to the animal's treatment and clinical status for evaluating the scans

positive controls

None specified

negative controls

None specified

Annotations

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