Light-induced histone modification was performed similarly to a previous report 33 (link), 35 (link). Briefly, cells were illuminated using a custom-built LED array aligned to a 6-well cell culture plate. LEDs were driven by a waveform generator (Rigol DG1022U) and powered by a DC power supply (Arksen 305D). Illumination was measured using a Thorlabs PM200 Power Meter and a S120C Power Sensor. The temperature inside the wells was measured using BMDS wireless temperature probes. Plates containing cells incubated in the dark were wrapped in aluminum foil. The following stimulation parameters were used for experiments: 466 nm, 5 mW/cm2 for 24 h. Pulses were delivered at 0.067 Hz with a duration of 7% corresponding to 1 s pulses.
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Light-Induced Histone Modification Protocol
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Corresponding Organization :
Other organizations : Tufts University, Zhejiang University, Second Affiliated Hospital of Zhejiang University
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Variable analysis
independent variables
- Illumination duration: 24 h
- Illumination wavelength: 466 nm
- Illumination intensity: 5 mW/cm^2
- Illumination pattern: Pulses at 0.067 Hz with a duration of 7% (1 s pulses)
- Histone modification (unspecified)
- Temperature inside the wells
- Cells incubated in the dark (wrapped in aluminum foil)
- Positive control: Not explicitly mentioned
- Negative control: Cells incubated in the dark (wrapped in aluminum foil)
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