Immunohistochemical Evaluation of HER2 in Colorectal Cancer

To begin with, 3 μm sections from the FFPE of 14 CRC patients’ biopsies were immunohistochemically stained using the rabbit monoclonal antibody for HER2 (1:4000 dilution; ab214275, Abcam, Waltham, MA, USA) according to the manufacturer’s instructions. An experienced pathologist (R.H.) scored the stained slides, following the consensus recommendations for HER2 scoring for CRC [17 (link),18 (link)]. Briefly, scoring was performed on a 4-point scale—0, 1+, 2+, 3+ focusing on intensity and extent according to the Allred scoring system [19 (link)]. In this study, 0 and 1+ intensity were taken to be negative for HER2 expression and the study focused on the assessment of membranous HER2 expression.

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Abdul Razzaq E.A., Bajbouj K., Bouzid A., Alkhayyal N., Hamoudi R, & Bendardaf R. (2022). Transcriptomic Changes Associated with ERBB2 Overexpression in Colorectal Cancer Implicate a Potential Role of the Wnt Signaling Pathway in Tumorigenesis. Cancers, 15(1), 130.

Publication 2022

ab214275

Biopsies Dilution Her2 Membranous Monoclonal antibody her2 Pathologist Patients Rabbit

Corresponding Organization : University College London

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Variable analysis

independent variables

Immunohistochemical staining using the rabbit monoclonal antibody for HER2 (1:4000 dilution; ab214275, Abcam, Waltham, MA, USA)

dependent variables

HER2 expression, assessed by an experienced pathologist (R.H.) on a 4-point scale (0, 1+, 2+, 3+) focusing on intensity and extent according to the Allred scoring system

control variables

3 μm sections from the FFPE of 14 CRC patients' biopsies

positive controls

None specified

negative controls

None specified

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