Heparinised peripheral blood samples were obtained for analysis of lymphocyte populations and subpopulations by flow cytometry. Whole blood was collected in sodium heparin vacutainers (Becton Dickinson) and shipped ambient overnight to the ITN Flow Cytometry Core (Roswell Park Cancer Institute). Using a stain-lyse method, cells from blinded samples were labelled with 5-colour monoclonal antibody panels using anti-human CD8−PE-Cy5, CD57-FITC (clone NK-1), CD56-PE (clone NCA-1) CD14-APC (clone MϕP9), plus CD3-PE-Cy7 (clone SK7, all BD Biosciences). Following staining, data were acquired on a FACSCanto flow cytometer (BD Biosciences), and analysed using WinList’s™ (http://www.vsh.com ) FCOM function40 (link).
A validated panel of KIR receptors22 (link) was used to quantify NK cell subset expression of activating and inhibitory receptors for which clone ids and reagent sources are detailed in Supplementary Data4 . Flow analysis was undertaken using an LSR Fortessa (BD) in the NIHR Cambridge BRC flow phenotyping hub (Supplementary Fig. 9 ).
A validated panel of KIR receptors22 (link) was used to quantify NK cell subset expression of activating and inhibitory receptors for which clone ids and reagent sources are detailed in Supplementary Data
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