Heteroclitic Peptides with Novel p4 Substitutions

Heteroclitic peptides (htcPep) with substitution in the p4 residue were described in our previously study (17 (link)). An additional htcPep was designed introducing at p4 a non-natural aa residue based on the Tryptophan structure (NAL). Alternatively, a peptide library including 20 peptides was prepared by random introduction of all 20 possible amino acids at position 4 (MIX). Individual htcPep were synthesized at a purity > 95% determined by LC-MS analyses. Lyophilized powder was dissolved in dimethylsulfoxide (DMSO; Sigma-Aldrich), diluted in phosphate-buffered saline (1× PBS; Gibco Life Technologies) and stored at − 80°C until use.