Compound Screening for Chagas Disease

Compounds were prepared in 100% DMSO, at the following concentrations: benznidazole (supplied by EpiChem Pty Ltd., Perth, Australia): 34.7 mM, nifurtimox (Sigma Aldrich, St. Louis, MO, USA): 34.7 mM, ciclopirox olamine: 40 mM, camptothecin 20 mM, clotrimazole 17.5 mM (all purchased from Sigma Aldrich, USA). Ethyl oxo{[2-(4-phenyl-1-piperazinyl)-2-(3-pyridinyl)ethyl]amino} acetate: 20 mM (E155-0206; Chemdiv, San Diego, CA, USA). MMV688958 and MMV688796 were sourced from Enamine (Kyiv, Ukraine) and prepared at 20 mM in 100% DMSO. Compounds were tested in assays in 14 log dilutions to determine IC₅₀ values, ranging from 73 µM to 3.7 × 10⁻³ µM for camptothecin, E155-0206, MMV688958 and MMV688796; 64.1 µM to 2.6 µM for clotrimazole; 146 µM to 2.6 µM for ciclopirox olamine and 127 µM to 6.4 × 10⁻³ µM for nifurtimox and benznidazole, to determine IC₅₀ values.