Collection and Maintenance of Starfish

Specimens of starfish (Asterias rubens) with a diameter > 4 cm were collected at low tide from the Thanet Coast (Kent, UK) or were obtained from a fisherman based at Whitstable (Kent, UK). The starfish were maintained in an aquarium with circulating seawater (salinity of 32 ‰) under a 12 h–12 h light–dark cycle (lights on at 8 a.m.) at a temperature of ~12 °C, located in the School of Biological & Behavioural Sciences at Queen Mary University of London. Animals were fed on mussels (Mytilus edulis) that were collected at low tide near Margate (Kent, UK). Additionally, juvenile specimens of A. rubens (diameter 0.5–1.5 cm) were collected from the University of Gothenburg Sven Lovén Centre for Marine Infrastructure (Kristineberg, Sweden).

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Tinoco A.B., Egertová M, & Elphick M.R. (2023). Immunohistochemical localisation of vasopressin/oxytocin-type, corazonin-type and luqin-type neuropeptide expression in the starfish Asterias rubens using antibodies to the C-terminal region of precursor proteins. Cell and Tissue Research, 391(3), 441-456.

Publication 2023

Animals Asterias Biological Lights Marine Mussels Mytilus edulis Rubens Salinity Starfish

Corresponding Organization :

Other organizations : Queen Mary University of London

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Variable analysis

independent variables

Size of starfish (diameter > 4 cm or 0.5-1.5 cm)

dependent variables

Unspecified

control variables

Salinity of seawater (32 ‰)
Light-dark cycle (12 h–12 h, lights on at 8 a.m.)
Temperature (~12 °C)

controls

Positive control: Juvenile specimens of A. rubens (diameter 0.5–1.5 cm) collected from the University of Gothenburg Sven Lovén Centre for Marine Infrastructure (Kristineberg, Sweden)
Negative control: Not specified

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