Immunohistochemical Analysis of Ki67 in Mice Tumors

We obtained the mice tumor tissues, which were fixed by using the 10% (v/v) formaldehyde and the tissues were subsequently embedded into the paraffin. Then, the above tissues were prepared as sections with about 5 μm thickness. After that, according to the protocols provided by the previous publication [34 (link)], we performed IHC assay to examine the expression status, including expression levels and localization of Ki67 protein in mice tumor tissues. Briefly, the tissues were sequentially incubated with primary antibody against Ki67 (1:400, #ab245113, Abcam, UK) and secondary antibody (Cell signaling Technology, USA), and the yellow Ki67-postive cells were observed and counted under a light microscope (ThermoFisher Scientific, USA).